Downregulation of adiponectin system in granulosa cells and low levels of HMW adiponectin in PCOS

Artimani, T. and Saidijam, M. and Aflatoonian, R. and Ashrafi, M. and Amiri, I. and Yavangi, M. and Soleimani Asl, S. and Shabab, N. and Karimi, J. and Mehdizadeh, M. (2016) Downregulation of adiponectin system in granulosa cells and low levels of HMW adiponectin in PCOS. Journal of Assisted Reproduction and Genetics, 33 (1). pp. 101-110.

Full text not available from this repository.
Official URL:


Purpose: The purpose of the study was to investigate changes in adiponectin system expression in granulosa cells (GCs) and high molecular weight adiponectin levels in serum and follicular fluid (FF) of 40 women with polycystic ovary syndrome (PCOS) compared to those in 40 women with normal ovary function. Methods: Adiponectin (Adipo), adiponectin receptor 1 (AdipoR1), and adiponectin receptor 2 (AdipoR2) messenger RNA (mRNA) expression levels were measured using quantitative real-time polymerase chain reaction (qRT-PCR). High molecular weight (HMW) adiponectin protein concentration was evaluated by ELISA method. Data were analyzed using Student�s t test and one-way ANOVA in SPSS 21 software. At oocyte retrieval, FF was aspirated and GCs were obtained from a pooled collection of FF per each patient. Results: PCR results showed expression of adiponectin, AdipoR1, AdipoR2, follicle-stimulating hormone receptor (FSHR), and luteinizing hormone receptor (LHR) in GCs. After controlling body mass index (BMI) values, qRT-PCR demonstrated a decreased expression of adiponectin system in GCs of PCOS patients compared to those in controls (p = 0.001). There was a strong positive correlation among AdipoR1 and AdipoR2 expression and also among FSH and LH receptor expression. (Both r = 0.8, p = 0.001). There were low levels of high molecular weight adiponectin in the serum of PCOS patients with controlled ovarian hyperstimulation (30.19 ± 4.3 ng/ml) compared to the controls (48.47 ± 5.9 ng/ml) and in the FF of PCOS patients with controlled ovarian hyperstimulation (7.86 ± 1.44 ng/ml) compared to the controls (14.22 ± 2.01 ng/ml; p = 0.02). Conclusions: Lower expression of adiponectin and its receptors in GCs might be an important manifestation in gonadotropin-stimulated PCOS patients which could influence the physiologic adiponectin roles such as interaction with insulin and LH in induction of GC gene expression. © 2015, Springer Science+Business Media New York.

Item Type: Article
Additional Information: cited By 8
Depositing User: eprints admin
Date Deposited: 02 Jul 2018 09:43
Last Modified: 02 Jul 2018 09:43

Actions (login required)

View Item View Item