Prevalence, and virulence determination of listeria monocytogenes strains isolated from clinical and non-clinical samples by multiplex polymerase chain reaction

Pournajaf, A. and Rajabnia, R. and Sedighi, M. and Kassani, A. and Moqarabzadeh, V. and Lotfollahi, L. and Ardebilli, A. and Emadi, B. and Irajian, G. (2016) Prevalence, and virulence determination of listeria monocytogenes strains isolated from clinical and non-clinical samples by multiplex polymerase chain reaction. Revista da Sociedade Brasileira de Medicina Tropical, 49 (5). pp. 624-627.

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Prevalence-and-virulence-determination-of-listeria-monocytogenes-strains-isolated-from-clinical-and-nonclinical-samples-by-multiplex-polymerase-chain-reaction2016Revista-da-Sociedade-Brasileira-de-Medicina-Tropica.pdf

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Abstract

Introduction: This study aimed to determine the prevalence, and virulence factors of Listeria monocytogenes isolated from various samples by multiplex polymerase chain reaction (MPCR). Methods: A total of 617 isolates were obtained and MPCR was employed for detection of the inlA, inlC, and inlJ genes. Results: L. monocytogenes was detected in 46 (7.45) of the 617 specimens. inlA, inlC, and inlJ were detected in 100, 76.26, and 71 isolates, respectively. Conclusions: This study validated MPCR in the analysis and rapid detection of L. monocytogenes. The role of the genes in pathogenesis of the strains can also be affirmed. © 2016, Sociedade Brasileira de Medicina Tropical. All rights reserved.

Item Type: Article
Additional Information: cited By 0
Subjects: QW Microbiology. Immunology
Depositing User: eprints admin
Date Deposited: 02 Jul 2018 09:01
Last Modified: 24 Nov 2019 06:37
URI: http://eprints.iums.ac.ir/id/eprint/4492

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