Rikhtegaran Tehrani, Z. and Azadmanesh, K. and Mostafavi, E. and Soori, S. and Azizi, M. and Khabiri, A. (2015) Development of an integrase-based ELISA for specific diagnosis of individuals infected with HIV. Journal of Virological Methods, 215-21. pp. 61-66.
Full text not available from this repository.Abstract
Currently, enzyme immunoassays (EIAs) are the most common immunological diagnostic methods that are used as the screening tool in HIV detection. Among all three major genes of HIV, the products of gag and env are usually used in EIAs (ELISAs and rapid tests). Hence, the presence of cross reacting antibodies against these antigens leads to the appearance of repetitive false positive results in screening tests. Re-testing the primary reactive samples with EIAs using other HIV antigens can considerably reduce the rate of false positive results. The products of pol gene may act as an appropriate candidate in this context. Integrase is a conserved and immunogenic product of HIV, encoded by the pol gene. The aim of this research was to determine the sensitivity and specificity of an ELISA detecting integrase antibodies. Recombinant integrase was produced in Escherichia coli to develop the integrase-based ELISA. Assay performance was evaluated by HIV positive and negative sera and an HIV panel of BBI (PRB-601). The sensitivity and specificity of assay was determined as 96.7 95% confidence interval: 91.3-98.9% and 100% 95% CI: 96.1-100%, respectively. High specificity of this assay may suggest its possible use in the detection of HIV. © 2015 Elsevier B.V.
| Item Type: | Article |
|---|---|
| Additional Information: | cited By 2 |
| Depositing User: | eprints admin |
| Date Deposited: | 02 Jul 2018 08:00 |
| Last Modified: | 02 Jul 2018 08:00 |
| URI: | http://eprints.iums.ac.ir/id/eprint/4956 |
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