Asadi, Y. and Gorjipour, F. and Behrouzifar, S. and Vakili, A. (2018) Irisin Peptide Protects Brain Against Ischemic Injury Through Reducing Apoptosis and Enhancing BDNF in a Rodent Model of Stroke. Neurochemical Research, 43 (8). pp. 1549-1560.
Full text not available from this repository.Abstract
Evidence has shown therapeutic potential of irisin in cerebral stroke. The present study aimed to assess the effects of recombinant irisin on the infarct size, neurological outcomes, blood�brain barrier (BBB) permeability, apoptosis and brain-derived neurotrophic factor (BDNF) expression in a mouse model of stroke. Transient focal cerebral ischemia was established by middle cerebral artery occlusion (MCAO) for 45 min and followed reperfusion for 23 h in mice. Recombinant irisin was administrated at doses of 0.1, 0.5, 2.5, 7.5, and 15 µg/kg, intracerebroventricularly (ICV), on the MCAO beginning. Neurological outcomes, infarct size, brain edema and BBB permeability were evaluated by modified neurological severity score (mNSS), 2,3,5-triphenyltetrazolium chloride (TTC) staining and Evans blue (EB) extravasation methods, respectively, at 24 h after ischemia. Apoptotic cells and BDNF protein were detected by TUNEL assay and immunohistochemistry techniques. The levels of Bcl-2, Bax and caspase-3 proteins were measured by immunoblotting technique. ICV irisin administration at doses of 0.5, 2.5, 7.5 and 15 µg/kg, significantly reduced infarct size, whereas only in 7.5 and 15 µg/kg improved neurological outcome (P < 0.001). Treatment with irisin (7.5 µg/kg) reduced brain edema (P < 0.001) without changing BBB permeability (P > 0.05). Additionally, irisin (7.5 µg/kg) significantly diminished apoptotic cells and increased BDNF immunoreactivity in the ischemic brain cortex (P < 0.004). Irisin administration significantly downregulated the Bax and caspase-3 expression and upregulated the Bcl-2 protein. The present study indicated that irisin attenuates brain damage via reducing apoptosis and increasing BDNF protein of brain cortex in the experimental model of stroke in mice. © 2018, Springer Science+Business Media, LLC, part of Springer Nature.
| Item Type: | Article |
|---|---|
| Additional Information: | cited By 1 |
| Uncontrolled Keywords: | brain derived neurotrophic factor; caspase 3; irisin; protein Bax; protein bcl x; recombinant irisin; recombinant protein; unclassified drug, animal experiment; animal model; animal tissue; apoptosis; Article; blood brain barrier; brain blood flow; brain circulation; brain cortex; brain edema; brain infarction size; brain ischemia; brain protection; cerebrovascular accident; controlled study; disease severity assessment; dose response; down regulation; drug dose comparison; drug mechanism; extravasation; immunoblotting; male; membrane permeability; middle cerebral artery occlusion; motor performance; mouse; mouse model; nonhuman; priority journal; protein expression; rodent model; scoring system; sensation; treatment outcome; upregulation |
| Subjects: | WL Nervous System |
| Depositing User: | eprints admin |
| Date Deposited: | 30 Dec 2018 09:35 |
| Last Modified: | 21 Aug 2019 10:12 |
| URI: | http://eprints.iums.ac.ir/id/eprint/5998 |
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