Elimination of mouse tumor cells from neonate spermatogonial cells utilizing cisplatin-entrapped folic acid-conjugated poly(Lactic-co-glycolic acid) nanoparticles in vitro

Shabani, R. and Ashjari, M. and Ashtari, K. and Izadyar, F. and Behnam, B. and Khoei, S. and Asghari-Jafarabadi, M. and Koruji, M. (2018) Elimination of mouse tumor cells from neonate spermatogonial cells utilizing cisplatin-entrapped folic acid-conjugated poly(Lactic-co-glycolic acid) nanoparticles in vitro. International Journal of Nanomedicine, 13. pp. 2943-2954.

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Background: Some male survivors of childhood cancer are suffering from azoospermia. In addition, spermatogonial stem cells (SSCs) are necessary for the improvement of spermatogenesis subsequent to exposure to cytotoxic agents such as cisplatin. Objective: The aim of this study was to evaluate the anticancer activity of cisplatin-loaded folic acid-conjugated poly(lactic-co-glycolic acid) (PLGA) nanoparticles (NPs) on mouse malignant cell line (EL4) and SSCs in vitro. Methods: SSCs were co-cultured with mouse malignant cell line (EL4) cells and divided into four culture groups: 1) control (cells were co-cultured in the culture medium), 2) co-cultured cells were treated with cisplatin (10 μg/mL), 3) co-cultured cells were treated with cisplatin-loaded folic acid-conjugated PLGA NPs, and 4) co-cultures were treated with folic acid-conjugated PLGA for 48 hours. The NPs were prepared, characterized, and targeted with folate. In vitro release characteristics, loading efficiency, and scanning electron microscopy and transmission electron microscopy images were studied. Cancer cells were assayed after treatment using flow cytometry and TUNEL assay. The co-cultures of SSCs and EL4 cells were injected into seminiferous tubules of the testes after treating with cis-diaminedichloroplatinum/PLGA NPs. Results: The mean diameter of PLGA NPs ranged between 150 and 250 nm. The number of TUNEL-positive cells increased, and the expression of Bax and caspase-3 were upregulated in EL4 cells in Group 4 compared with Group 2. There was no pathological tumor in testes after transplantation with treated co-cultured cells. Conclusion: The PLGA NPs appeared to act as a promising carrier for cisplatin administration, which was consistent with a higher activation of apoptosis than free drug. © 2018 Shabani et al.

Item Type: Article
Additional Information: cited By 1
Uncontrolled Keywords: caspase 3; cisplatin; folic acid; nanoparticle; polyglactin; protein Bax; antineoplastic agent; cisplatin; drug carrier; folic acid; lactic acid; nanoparticle; polyglycolic acid; polylactic acid-polyglycolic acid copolymer, abdominal tumor; animal cell; antineoplastic activity; apoptosis; Article; Bax gene; cancer survival; cell proliferation; cell viability; controlled study; cytotoxicity; dispersion; down regulation; drug delivery system; drug distribution; drug efficacy; drug release; excipient compatibility; flow cytometry; gene expression; IC50; in vitro study; in vivo study; male; mouse; nanoencapsulation; newborn; nonhuman; particle size; physical chemistry; protein expression; scanning electron microscopy; spermatogonium; transmission electron microscopy; tumor cell; TUNEL assay; upregulation; zeta potential; animal; chemistry; coculture; drug effect; pathology; seminiferous tubule; spermatogonium; tumor cell line, Animals; Antineoplastic Agents; Apoptosis; Cell Line, Tumor; Cisplatin; Coculture Techniques; Drug Carriers; Folic Acid; Lactic Acid; Male; Mice; Microscopy, Electron, Transmission; Nanoparticles; Polyglycolic Acid; Seminiferous Tubules; Spermatogonia
Subjects: QT Physiology
WS Pediatrics
QU Biochemistry. Cell Biology and Genetics
Depositing User: eprints admin
Date Deposited: 31 Dec 2018 07:37
Last Modified: 24 Aug 2019 04:55
URI: http://eprints.iums.ac.ir/id/eprint/6031

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