Variable localization of Toll-like receptors in human fallopian tube epithelial cells

Amjadi, F. and Zandieh, Z. and Salehi, E. and Jafari, R. and Ghasemi, N. and Aflatoonian, A. and Fazeli, A. and Aflatoonian, R. (2018) Variable localization of Toll-like receptors in human fallopian tube epithelial cells. Clinical and Experimental Reproductive Medicine, 45 (1). pp. 1-9.


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Objective: To determine the localization, expression, and function of Toll-like receptors (TLRs) in fallopian tube epithelial cells. Methods: The localization of TLRs in fallopian tube epithelial cells was investigated by immunostaining. Surprisingly, the intensity of staining was not equal in the secretory and ciliated cells. After primary cell culture of fallopian tube epithelial cells, ring cloning was used to isolate colonies of ciliated epithelial cells, distinct from non-ciliated epithelial cells. The expression of TLRs 1-10 was examined by quantitative real-time polymerase chain reaction, and protein localization was confirmed by immunostaining. The function of the TLRs was determined by interleukin (IL)-6 and IL-8 production in response to TLR2, TLR3, TLR5, TLR7, and TLR9 ligands. Results: Fallopian tube epithelial cells expressed TLRs 1-10 in a cell-type-specific manner. Exposing fallopian tube epithelial cells to TLR2, TLR3, TLR5, TLR7, and TLR9 agonists induced the secretion of proinflammatory cytokines such as IL-6 and IL-8. Conclusion: Our findings suggest that TLR expression in the fallopian tubes is cell-type-specific. According to our results, ciliated cells may play more effective role than non-ciliated cells in the innate immune defense of the fallopian tubes, and in interactions with gametes and embryos. © 2018. The Korean Society For Reproductive Medicine.

Item Type: Article
Additional Information: cited By 0
Subjects: WP Gynecology
Depositing User: eprints admin
Date Deposited: 05 Aug 2018 04:44
Last Modified: 07 Sep 2019 09:51

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