Shahbazi, S. and Asadi Karam, M.R. and Habibi, M. and Talebi, A. and Bouzari, S. (2018) Distribution of extended-spectrum β-lactam, quinolone and carbapenem resistance genes, and genetic diversity among uropathogenic Escherichia coli isolates in Tehran, Iran. Journal of Global Antimicrobial Resistance, 14. pp. 118-125.
Full text not available from this repository.Abstract
Objectives: Antimicrobial resistance among uropathogenic Escherichia coli (UPEC) is increasing. The aim of this study was to evaluate antimicrobial resistance in UPEC isolates and its correlation with virulence factors. Methods: A total of 120 E. coli isolates were collected from patients with urinary tract infection in Tehran, Iran. Biofilm formation and antimicrobial susceptibility were determined by phenotypic tests. The combination disk diffusion test and modified Hodge test (MHT) were performed for phenotypic detection of extended-spectrum β-lactamases (ESBLs) and carbapenemases, respectively. PCR was used for the detection of virulence genes, ESBL-encoding genes, and quinolone (qnr) and carbapenem resistance genes. Pulsed-field gel electrophoresis (PFGE) was performed to explore the genetic relatedness among isolates. Results: Most isolates exhibited biofilm formation, and different frequencies of virulence genes were observed. There was a high rate of resistance, especially multidrug resistance, to most of the antimicrobial agents tested. Phenotypically, 67.5 of the isolates produced an ESBL and were resistant to different antimicrobial classes. In total, 83.3 of the isolates harboured ESBL genes, especially blaTEM and blaCTX-M, and 32.5 were positive for the quinolone resistance genes qnrS and qnrB. All isolates were negative for carbapenemases by the MHT and PCR. These results indicate the association between the presence of various virulence genes and antimicrobial susceptibility. PFGE analysis showed that there was great clonal diversity among the selected isolates, with 17 isolates divided into five clusters. Conclusions: These results indicate that the high antimicrobial resistance among UPEC isolates is alarming and requires urgent attention. © 2018 International Society for Chemotherapy of Infection and Cancer
| Item Type: | Article |
|---|---|
| Additional Information: | cited By 0 |
| Uncontrolled Keywords: | adhesin; amikacin; ampicillin; aztreonam; bacterial toxin; beta lactam antibiotic; beta lactamase; beta lactamase CTX M; carbapenem; carbapenemase; cefepime; cefixime; cefotaxime; ceftazidime; ceftriaxone; ciprofloxacin; clavulanic acid; cotrimoxazole; ertapenem; extended spectrum beta lactamase; genomic DNA; gentamicin; imipenem; iron; kanamycin; nalidixic acid; nitrofurantoin; norfloxacin; quinolone; virulence factor, antibiotic resistance; antibiotic sensitivity; Article; bacterial gene; bacterial virulence; biofilm; controlled study; disk diffusion; Escherichia coli; genetic variability; Iran; Klebsiella pneumoniae; multidrug resistance; nonhuman; priority journal; pulsed field gel electrophoresis; uropathogenic Escherichia coli; zone of inhibition |
| Subjects: | QZ Pathology |
| Depositing User: | eprints admin |
| Date Deposited: | 24 Dec 2018 11:15 |
| Last Modified: | 19 Aug 2019 08:41 |
| URI: | http://eprints.iums.ac.ir/id/eprint/6261 |
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