Species identification and in vitro antifungal susceptibility testing of Aspergillus section Nigri strains isolated from otomycosis patients

Kamali Sarwestani, Z. and Hashemi, S.J. and Rezaie, S. and Gerami Shoar, M. and Mahmoudi, S. and Elahi, M. and Bahardoost, M. and Tajdini, A. and Abutalebian, S. and Daie Ghazvini, R. (2018) Species identification and in vitro antifungal susceptibility testing of Aspergillus section Nigri strains isolated from otomycosis patients. Journal de Mycologie Medicale, 28 (2). pp. 279-284.

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Introduction: Aspergillus niger is the most commonly reported etiology of otomycosis based on morphological characteristics. This fungus is a member of Aspergillus section Nigri, a set of morphologically indistinguishable species that can harbor various antifungal susceptibility patterns. The aim of this study was to accurately identify and determine the susceptibility pattern of a set of black aspergilli isolated from otomycosis patients. Methods: Forty-three black Aspergillus isolates from otomycosis patients were identified by using the PCR-sequencing of the β-tubulin gene. Furthermore, the susceptibility of isolates to three antifungal drugs, including fluconazole (FLU), clotrimazole (CLT) and nystatin (NS), were tested according to CLSI M38-A2. The data were analyzed using the SPSS software (version 15). Results: The majority of isolates were identified as A. tubingensis (32/43, 74.42) followed by A. niger (11/43, 25.58). The lowest minimum inhibitory concentration (MIC) values were observed for NS with geometric means (GM) of 4.65 μg/mL and 4.83 μg/mL against A. tubingensis and A. niger isolates, respectively. CLT showed wide MIC ranges and a statistically significant inter-species difference was observed between A. tubingensis and A. niger isolates (P < 0.05). FLU was inactive against both species with GMs > 64 μg/mL. Conclusion: Species other than A. niger can be more frequent as observed in our study. In addition, considering the low and variable activity of tested antifungal drugs, empirical treatment can result in treatment failure. Accurate identification and antifungal susceptibility testing of isolates is, however, recommended. © 2018 Elsevier Masson SAS

Item Type: Article
Additional Information: cited By 0
Uncontrolled Keywords: beta tubulin; clotrimazole; fluconazole; nystatin, adolescent; adult; aged; antifungal susceptibility; Article; Aspergillus niger; Aspergillus tubingensis; clinical article; comparative study; controlled study; female; fungal strain; gene sequence; human; in vitro study; male; MIC50; MIC90; middle aged; minimum inhibitory concentration; otomycosis; pH; phylogeny; species identification
Subjects: WV Otolaryngology
Depositing User: eprints admin
Date Deposited: 22 Dec 2018 07:09
Last Modified: 22 Dec 2018 07:09
URI: http://eprints.iums.ac.ir/id/eprint/6584

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