High avidity anti-integrase antibodies discriminate recent and non-recent HIV infection: Implications for HIV incidence assay

Rikhtegaran Tehrani, Z. and Azadmanesh, K. and Mostafavi, E. and Gharibzadeh, S. and Soori, S. and Azizi, M. and Khabiri, A. (2018) High avidity anti-integrase antibodies discriminate recent and non-recent HIV infection: Implications for HIV incidence assay. Journal of Virological Methods, 253. pp. 5-10.

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Estimation of HIV incidence provides real-time information of HIV transmission trends for decision makers. Anti-integrase antibodies are the last ones produced during seroconversion and presence of high-avidity anti-integrase antibodies indicates the chronicity of HIV infection. This study aimed to evaluate the performance of these antibodies in discriminating of recent from non-recent HIV infection. For this purpose, different ELISA formats were developed to detect high-avidity anti-integrase antibodies in a commercially available performance panel, and the best assay was selected for further evaluation. The false recent rate of the selected assay was evaluated in a panel of Iranian patients and compared to two commercial assays, BED-EIA and LAg-Avidity. While the false recent rate of the developed assay was 3.8, it was 14.1 and 1.3 for BED-EIA and LAg-Avidity, respectively. To our knowledge, this is the first report to study the performance of high-avidity anti-integrase antibodies for classification of HIV infection. The preliminary results showed that the specificity of the newly developed assay is markedly higher than BED-EIA and is comparable with LAg-Avidity. The promising results point to the potential use of anti-integrase antibodies as a biomarker in HIV incidence laboratory tests or algorithms. The developed assay needs further evaluation in future. © 2017

Item Type: Article
Additional Information: cited By 0
Uncontrolled Keywords: enzyme antibody; immunoglobulin G antibody; integrase antibody; unclassified drug, adult; antibody detection; Article; binding affinity; controlled study; double antibody sandwich enzyme linked immunosorbent assay; enzyme immunoassay; enzyme linked immunosorbent assay; female; human; Human immunodeficiency virus 1 infection; incidence; intermethod comparison; Iranian (citizen); major clinical study; male; prevalence; priority journal
Subjects: WC Communicable Diseases
WH Hemic and Lymphatic Systems
Depositing User: eprints admin
Date Deposited: 12 Dec 2018 14:31
Last Modified: 14 Aug 2019 10:51
URI: http://eprints.iums.ac.ir/id/eprint/6842

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