A comparative study between platelet-rich plasma and platelet-poor plasma effects on angiogenesis

Shahidi, M. and Vatanmakanian, M. and Arami, M.K. and Sadeghi Shirazi, F. and Esmaeili, N. and Hydarporian, S. and Jafari, S. (2018) A comparative study between platelet-rich plasma and platelet-poor plasma effects on angiogenesis. Medical Molecular Morphology, 51 (1). pp. 21-31.

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Platelet-rich plasma (PRP) has been established as an autologous source for therapeutic angiogenesis. The purpose of this study was to evaluate PRP angiogenic effects compared to platelet-poor plasma (PPP) in vitro and in vivo. The effects of PRP on vascular endothelial growth factor receptor-2 (VEGFR2) and CD34 expression were evaluated using real-time PCR, flow cytometry, western blot, immunocytochemistry and pathological study, as were carried out in both human umbilical endothelial cell culture and rat skin. Our findings indicated significant effect of PRP and PPP on VEGFR2 and CD34 expression by human umbilical vein endothelial cells, which was greater in latter. These effects, however, were confirmed by demonstrating an earlier angiogenic effect of PPP in vivo when compared to PRP. The findings of the present study as the first comparative study of PRP versus PPP are novel. Nevertheless, further studies are needed to clarify the underlying mechanism of these findings to improve the therapeutic effects of PRP and PPP. © 2017, The Japanese Society for Clinical Molecular Morphology.

Item Type: Article
Additional Information: cited By 0
Uncontrolled Keywords: CD34 antigen; immunoglobulin enhancer binding protein; vasculotropin receptor 2; CD34 antigen; vasculotropin receptor 2, angiogenesis; Article; cell cycle; comparative study; extraction; flow cytometry; gene expression; human; human cell; HUVEC cell line; immunocytochemistry; immunophenotyping; inflammation; priority journal; real time polymerase chain reaction; thrombocyte poor plasma; thrombocyte rich plasma; Western blotting; animal; blood; cell proliferation; genetics; metabolism; neovascularization (pathology); pathology; rat; thrombocyte; thrombocyte rich plasma; umbilical vein endothelial cell, Animals; Antigens, CD34; Blood Platelets; Cell Proliferation; Flow Cytometry; Human Umbilical Vein Endothelial Cells; Humans; Neovascularization, Pathologic; Platelet-Rich Plasma; Rats; Vascular Endothelial Growth Factor Receptor-2
Subjects: WH Hemic and Lymphatic Systems
Depositing User: eprints admin
Date Deposited: 12 Dec 2018 11:13
Last Modified: 14 Aug 2019 10:02
URI: http://eprints.iums.ac.ir/id/eprint/6873

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