The antimicrobial potential of a new derivative of cathelicidin from Bungarus fasciatus against methicillin-resistant Staphylococcus aureus

Tajbakhsh, M. and Karimi, A. and Tohidpour, A. and Abbasi, N. and Fallah, F. and Akhavan, M.M. (2018) The antimicrobial potential of a new derivative of cathelicidin from Bungarus fasciatus against methicillin-resistant Staphylococcus aureus. Journal of Microbiology, 56 (2). pp. 128-137.

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Cathelicidins are a family of antimicrobial peptides which exhibit broad antimicrobial activities against antibiotic-resistant bacteria. Considering the progressive antibiotic resistance, cathelicidin is a candidate for use as an alternative approach to treat and overcome the challenge of antimicrobial resistance. Cathelicidin-BF (Cath-BF) is a short antimicrobial peptide, which was originally extracted from the venom of Bungarus fasciatus. Recent studies have reported that Cath-BF and some related derivatives exert strong antimicrobial and weak hemolytic properties. This study investigates the bactericidal and cytotoxic effects of Cath-BF and its analogs (Cath-A and Cath-B). Cath-A and Cath-B were designed to increase their net positive charge, to have more activity against methicillin resistant S. aureus (MRSA). The results of this study show that Cath-A, with a +17-net charge, has the most noteworthy antimicrobial activity against MRSA strains, with minimum inhibitory concentration (MIC) ranging between 32�128 μg/ml. The bacterial kinetic analysis by 1 � MIC concentration of each peptide shows that Cath-A neutralizes the clinical MRSA isolate for 60 min. The present data support the notion that increasing the positive net charge of antimicrobial peptides can increase their potential antimicrobial activity. Cath-A also displayed the weakest cytotoxicity effect against human umbilical vein endothelial and H9c2 rat cardiomyoblast cell lines. Analysis of the hemolytic activity reveals that all three peptides exhibit minor hemolytic activity against human erythrocytes at concentrations up to 250 μg/ml. Altogether, these results suggest that Cath-A and Cath-B are competent candidates as novel antimicrobial compounds against MRSA and possibly other multidrug resistant bacteria. © 2018, The Microbiological Society of Korea and Springer-Verlag GmbH Germany, part of Springer Nature.

Item Type: Article
Additional Information: cited By 0
Uncontrolled Keywords: antiinfective agent; antimicrobial cationic peptide; CAP18 lipopolysaccharide-binding protein; cathelicidin; cathelicidin BF, Bungarus fasciatus; peptide; reactive oxygen metabolite; venom, amino acid sequence; animal; antibiotic resistance; Bungarus; cell line; cell survival; chemistry; drug effect; hemolysis; human; Iran; kinetics; metabolism; methicillin resistant Staphylococcus aureus; microbial sensitivity test; multidrug resistance; protein conformation; rat, Amino Acid Sequence; Animals; Anti-Infective Agents; Antimicrobial Cationic Peptides; Bungarus; Cathelicidins; Cell Line; Cell Survival; Drug Resistance, Microbial; Drug Resistance, Multiple, Bacterial; Hemolysis; Humans; Iran; Kinetics; Methicillin-Resistant Staphylococcus aureus; Microbial Sensitivity Tests; Peptides; Protein Conformation; Rats; Reactive Oxygen Species; Venoms
Subjects: WR Dermatology
Depositing User: eprints admin
Date Deposited: 11 Dec 2018 15:29
Last Modified: 14 Aug 2019 09:29

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