Molecular characterization of hepatitis D virus genotypes circulating in Iran

Meysami, P. and Alavian, S.M. and Monavari, S.H. and Mozhgani, S.-H. and Taghizadeh, M. and Farahani, M. and Ansari, N. and Keyvani, H. (2018) Molecular characterization of hepatitis D virus genotypes circulating in Iran. Future Virology, 13 (4). pp. 265-273.

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Aim: To determine the molecular epidemiology and characterization of hepatitis D virus (HDV) genotypes circulating in different provinces of Iran. Patients & methods: In this study, the presence of HDV RNA was tested in sera that were positive for hepatitis B surface antigen and HDV antibody by nested-PCR. HDV genotypes were subsequently analyzed using restriction fragment length polymorphism (RFLP) assay and then confirmed by sequencing. Results: 86.5 of positive PCR patients had genotype I and 8.1 had genotype II while the genotype of 5.4 of the patients remained undetermined by RFLP. Sequencing followed by phylogenetic analysis demonstrated that all the Iranian isolates were from genotype I. Conclusion: Although analyzing the RFLP of RT-PCR is a simpler method, the gold standard of genotyping of HDV is the phylogenetic analysis based on sequencing. © 2018 Future Medicine Ltd.

Item Type: Article
Additional Information: cited By 0
Uncontrolled Keywords: hepatitis B surface antigen; protein kinase R; virus antibody; virus RNA, adult; Article; cladistics; cross-sectional study; delta agent hepatitis; female; gene sequence; genetic distance; genotype; Hepatitis delta virus; Hepatitis delta virus genotype 1; Hepatitis delta virus genotype 2; Hepatitis delta virus genotype 3; Hepatitis delta virus genotype 4; Hepatitis delta virus genotype 5; Hepatitis delta virus genotype 6; Hepatitis delta virus genotype 7; Hepatitis delta virus genotype 8; human; Iran; major clinical study; male; middle aged; molecular epidemiology; nested polymerase chain reaction; nonhuman; nucleotide sequence; phylogeny; priority journal; restriction fragment length polymorphism; reverse transcription polymerase chain reaction; sequence analysis
Subjects: WH Hemic and Lymphatic Systems
Depositing User: eprints admin
Date Deposited: 08 Dec 2018 06:52
Last Modified: 08 Dec 2018 06:52

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