Detection and quantification of Streptococcus pneumoniae from Iranian patients with pneumonia and individual carriers by real time PCR

Nomanpour, B. and Ghodousi, A. and Babaei, T. and Mousavi, S.A. and Asadi, S. and Feizabadi, M.M. (2011) Detection and quantification of Streptococcus pneumoniae from Iranian patients with pneumonia and individual carriers by real time PCR. African Journal of Biotechnology, 10 (60). pp. 12826-12832.

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The aim of this study was to develop a real time polymerase chain reaction (PCR) for quantitative detection of Streptococcus pneumoniae from clinical respiratory specimens. Initially, 184 respiratory specimens from patients with community acquired pneumonia (CAP) (n = 129) and 55 cases with hospital associated pneumonia (HAP) were bacteriologically investigated. To check the colonization status among the healthy individuals, 32 preschool and 31 adults were screened in parallel. All specimens were cultured on selective culture media to isolate S. pneumoniae, Legionella spp. and Mycoplasma spp. A 166 bp fragment corresponding to cbp A gene of S. pneumoniae was amplified from clinical specimens using Taqman probe real time PCR. Culture showed 14, but real time PCR showed 15 specimens as being positive for S. pneumoniae. The specificity and sensitivity of real time PCR was 99.14 and 100 respectively. Co-infections of S. pneumoniae with Legionella pneumophila, Chlamydophila pneumoniae, Mycoplasma pneumoniae and Staphylococcus aureus were observed in 5 cases (35.72). S. pneumoniae was counted <10 3 cfu/ml from the co-infected cases. Using real time PCR, a cutoff of 10 3cfu/ml is introduced to differentiate colonization from infection in respiratory tract. This is the first report on the prevalence CAP with S. pneumoniae in Iran (12.40). © 2011 Academic Journals.

Item Type: Article
Additional Information: cited By 1
Uncontrolled Keywords: adolescent; adult; aged; article; bacterial colonization; bacterial gene; bacterium culture; bacterium detection; bacterium isolate; cbp A gene; child; Chlamydophila pneumoniae; community acquired pneumonia; controlled study; cross-sectional study; ethnic group; gene amplification; hospital acquired pneumonia; human; Iranian; Legionella pneumophila; major clinical study; mixed infection; Mycoplasma pneumoniae; nonhuman; nucleotide sequence; preschool child; quantitative analysis; real time polymerase chain reaction; respiratory tract infection; school child; screening; sensitivity and specificity; Staphylococcus aureus; Streptococcus pneumoniae, Chlamydophila pneumoniae; Legionella; Legionella pneumophila; Mycoplasma; Mycoplasma pneumoniae; Staphylococcus aureus; Streptococcus pneumoniae
Subjects: WF Respiratory System
Depositing User: somayeh pourmorteza
Date Deposited: 05 Jan 2019 09:50
Last Modified: 05 Jan 2019 09:50

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