Fahimzad, A. and Afgeh, S.A. and Eghbali, E. and Abdinia, B. and Shiva, F. and Rahbar, M. (2013) Screening of congenital CMV infection in saliva of neonates by PCR: Report of a pilot screening study in Iran. Clinical Laboratory, 59 (9-10). pp. 1171-1174.
Full text not available from this repository.Abstract
Background: Cytomegalovirus (CMV) is a leading cause of congenital infection in neonates. Most infants with congenital CMV infection are asymptomatic at birth and not diagnosed on routine clinical examination. To identify these at-risk infants early in life, polymerase chain reaction (PCR) assays are done to screen large populations of newborn infants. Objective: We carried out a pilot study to estimate the prevalence of CMV in saliva from newborns by DNA PCR assay. Methods: This study was performed from January 2012 to March 2012 at a maternity hospital in the south of Tehran. All newborns aged between 1 to 14 days born at this hospital were enrolled. Saliva specimens from newborns were collected by swabbing the inside of the baby's mouth and stored at -70°C until PCR processing for virus detection. Results: Six-hundred and twenty infants between 1 to 14 days of age were enrolled during the study period of two months. The PCR assay was positive for CMV in 2 newborns 0.3%. Both of these infants were asymptomatic for congenital CMV at birth and also when followed up at three months and six months of age. Conclusions: Our findings reveal that because of a low yield of positive results, screening for congenital CMV infection would not be cost-effective in Iranian neonates.
| Item Type: | Article |
|---|---|
| Additional Information: | cited By 2 |
| Uncontrolled Keywords: | DNA, article; congenital infection; controlled study; cytomegalovirus infection; DNA determination; female; human; Iran; major clinical study; male; newborn; newborn screening; pilot study; prevalence; saliva; virus detection, Base Sequence; Cytomegalovirus; Cytomegalovirus Infections; DNA Primers; DNA, Viral; Female; Humans; Infant, Newborn; Iran; Male; Neonatal Screening; Pilot Projects; Polymerase Chain Reaction; Saliva |
| Subjects: | WS Pediatrics |
| Depositing User: | somayeh pourmorteza |
| Date Deposited: | 29 May 2019 13:42 |
| Last Modified: | 29 May 2019 13:42 |
| URI: | http://eprints.iums.ac.ir/id/eprint/9568 |
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