Screening of congenital CMV infection in saliva of neonates by PCR: Report of a pilot screening study in Iran

Fahimzad, A. and Afgeh, S.A. and Eghbali, E. and Abdinia, B. and Shiva, F. and Rahbar, M. (2013) Screening of congenital CMV infection in saliva of neonates by PCR: Report of a pilot screening study in Iran. Clinical Laboratory, 59 (9-10). pp. 1171-1174.

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Background: Cytomegalovirus (CMV) is a leading cause of congenital infection in neonates. Most infants with congenital CMV infection are asymptomatic at birth and not diagnosed on routine clinical examination. To identify these at-risk infants early in life, polymerase chain reaction (PCR) assays are done to screen large populations of newborn infants. Objective: We carried out a pilot study to estimate the prevalence of CMV in saliva from newborns by DNA PCR assay. Methods: This study was performed from January 2012 to March 2012 at a maternity hospital in the south of Tehran. All newborns aged between 1 to 14 days born at this hospital were enrolled. Saliva specimens from newborns were collected by swabbing the inside of the baby's mouth and stored at -70°C until PCR processing for virus detection. Results: Six-hundred and twenty infants between 1 to 14 days of age were enrolled during the study period of two months. The PCR assay was positive for CMV in 2 newborns 0.3%. Both of these infants were asymptomatic for congenital CMV at birth and also when followed up at three months and six months of age. Conclusions: Our findings reveal that because of a low yield of positive results, screening for congenital CMV infection would not be cost-effective in Iranian neonates.

Item Type: Article
Additional Information: cited By 2
Uncontrolled Keywords: DNA, article; congenital infection; controlled study; cytomegalovirus infection; DNA determination; female; human; Iran; major clinical study; male; newborn; newborn screening; pilot study; prevalence; saliva; virus detection, Base Sequence; Cytomegalovirus; Cytomegalovirus Infections; DNA Primers; DNA, Viral; Female; Humans; Infant, Newborn; Iran; Male; Neonatal Screening; Pilot Projects; Polymerase Chain Reaction; Saliva
Subjects: WS Pediatrics
Depositing User: somayeh pourmorteza
Date Deposited: 29 May 2019 13:42
Last Modified: 29 May 2019 13:42

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