Delbandi, A.-A. and Mahmoudi, M. and Shervin, A. and Akbari, E. and Jeddi-Tehrani, M. and Sankian, M. and Kazemnejad, S. and Zarnani, A.-H. (2013) Eutopic and ectopic stromal cells from patients with endometriosis exhibit differential invasive, adhesive, and proliferative behavior. Fertility and Sterility, 100 (3). pp. 761-769.
Full text not available from this repository.Abstract
Objective: To study immunophenotype, differential proliferation capacity, invasiveness, adhesion, and cytokine production in ectopic and eutopic endometrial stromal cells (EESCs and EuESCs) from patients with endometriosis. Design: In vitro study. Setting: Academic research center. Patient(s): Patients with ovarian endometriosis (endometrioma) and nonendometriotic controls. Intervention(s): None. Main Outcome Measure(s): EESCs and EuESCs from 25 patients with endometrioma and ESCs from 20 nonendometriotic controls (CESCs) were isolated, and their immunophenotype, proliferation, invasion, adhesion, and cytokine production were assessed and compared. Result(s): Isolated ESCs from all three sources expressed markers specific for cells of mesenchymal origin but were negative for hematopoietic markers. EESCs exhibited a significantly lower proliferation rate in fibronectin-coated plates and less invasive capacity compared with CESCs or EuESCs. Among all stromal cell groups studied, EuESCs showed the highest invasive behavior. EESCs adhered more firmly to extracellular matrix than EuESCs or CESCs in all time intervals examined. The levels of interleukin (IL) -6 and IL-8 production by EESCs were significantly higher compared with those of EuESCs or CESCs. Conclusion(s): The results of the present study demonstrated that retrograde menstruation alone does not account for the pathogenesis of endometriosis as eutopic and ectopic counterparts of ESCs from patients with endometriosis exhibit differential invasive, adhesive, and proliferative behavior. © 2013 by American Society for Reproductive Medicine.
| Item Type: | Article |
|---|---|
| Additional Information: | cited By 28 |
| Uncontrolled Keywords: | interleukin 10; interleukin 17; interleukin 6; interleukin 8; transforming growth factor beta; tumor necrosis factor alpha, adult; article; cell adhesion; cell differentiation; cell invasion; cell proliferation; clinical article; controlled study; cytokine production; endometriosis; endometrium cell; endometrium tumor; extracellular matrix; female; human; human cell; immunophenotyping; in vitro study; priority journal; stroma cell, adhesion; cytokine; Endometrioma; eutopic; immunophenotype; invasion; proliferation, Adult; Cell Adhesion; Cell Movement; Cell Proliferation; Cells, Cultured; Choristoma; Cytokines; Endometriosis; Endometrium; Female; Humans; Middle Aged; Stromal Cells; Young Adult |
| Subjects: | WP Gynecology |
| Depositing User: | somayeh pourmorteza |
| Date Deposited: | 26 May 2019 07:07 |
| Last Modified: | 26 May 2019 07:07 |
| URI: | http://eprints.iums.ac.ir/id/eprint/9636 |
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