Keyvani, H. and Bokharaei-Salim, F. and Monavari, S.H. and Esghaei, M. and Toosi, M.N. and Fakhim, S. and Sadigh, Z.-A. and Alavian, S.M. (2013) Occult hepatitis C virus infection in candidates for liver transplant with cryptogenic cirrhosis. Hepatitis Monthly, 13 (8). p. 2.
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Abstract
Background: Occult hepatitis C virus (HCV) infection is a new entity described by the presence of HCV-RNA in liver biopsy and/or peripheral blood mononuclear cell (PBMC) specimens, and undetectable levels or absence of HCV-RNA and in the absence or presence of anti HCV antibodies in plasma by current laboratory methods. Objectives: To evaluate the detection of HCV-RNA in PBMC specimens of the liver transplant candidates with cryptogenic cirrhosis by reverse transcriptase-nested polymerase chain reaction (RT-nested PCR). Patients and Methods: From November 2007 to March 2013, 45 patients from Liver Transplant Center of Imam Khomeini Hospital, Tehran, were enrolled in this cross sectional study. PBMC specimens were separated from the peripheral blood sample. After extraction of RNA from plasma and PBMC specimens, HCV-RNA status was tested by RT-nested PCR. The 5�-untranslated region (5�-UTR) genotyping of HCV-RNA amplified from PBMC specimens was performed by a standard methodology with the INNO-LiPATM HCV II kit. The PCR products of 5�-UTR were sequenced after cloning into the pJET1.2 / blunt cloning vector. Results: Of 45 patients, 4 (8.9 95% CI: 4.4-15.6) had detectable genomic HCV-RNA in their PBMC specimens. HCV genotypes were determined in the PBMCs of these subjects showed that 2 (50.0%) subjects with occult HCV infection had HCV subtype 3a, and 2 (50.0%) had HCV subtype 1b. Conclusions: This study found that 8.9 % of the Iranian candidates for liver transplant with cryptogenic cirrhosis had occult HCV infection. Therefore, designing prospective studies focusing on the diagnosis of occult HCV infection in these subjects prior to liver transplantation could be valuable. © 2013, Kowsar Corp.; Licensee Kowsar Ltd.
| Item Type: | Article |
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| Additional Information: | cited By 24 |
| Uncontrolled Keywords: | alanine aminotransferase; virus RNA, 5' untranslated region; adult; article; clinical article; controlled study; cross-sectional study; enzyme immunoassay; enzyme linked immunosorbent assay; female; genotype; hepatitis C; human; human cell; liver cirrhosis; liver graft; male; neighbor joining method; nucleotide sequence; occult blood; peripheral blood mononuclear cell; reverse transcription polymerase chain reaction; RNA isolation; sensitivity analysis; sequence analysis; Western blotting |
| Subjects: | WC Communicable Diseases WI Digestive System |
| Depositing User: | somayeh pourmorteza |
| Date Deposited: | 19 May 2019 06:31 |
| Last Modified: | 19 May 2019 06:31 |
| URI: | http://eprints.iums.ac.ir/id/eprint/9659 |
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